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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9828

Title: Synthetic diamond films as a platform material for label-free protein sensors
Authors: BIJNENS, Nathalie
VERMEEREN, Veronique
DAENEN, Michael
GRIETEN, Lars
HAENEN, Ken
WENMACKERS, Sylvia
WILLIAMS, Oliver
AMELOOT, Marcel
VAN DE VEN, Martin
MICHIELS, Luc
WAGNER, Patrick
Issue Date: 2009
Publisher: WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY
Citation: PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 206(3). p. 520-526
Abstract: In the framework of developing a fast and label-free immunosensor for C-reactive protein (CRP) detection, H-terminated nanocrystalline diamond (NCD) was functionalised with anti-CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real-time CRP recognition. Different impedance behaviours were observed after CRP addition as compared to after FITC-labelled ssDNA addition at low (100 Hz) as well as at high frequencies (1 MHz). Physical interpretations of the observed impedance changes were obtained by fitting the data to an equivalent electrical circuit. Concentrations of 1 mu M CRP were recognised with a reaction time of 30 minutes.
URI: http://hdl.handle.net/1942/9828
DOI: 10.1002/pssa.200880485
ISI #: 000264691800018
ISSN: 1862-6300
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Bio-Physics
Physiology
Immunology - Biochemistry
Biomedical Research Institute
Institute for Materials Research
Materials Physics

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