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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/924

Title: DNA attachment to nanocrystalline diamond films
Authors: WENMACKERS, Sylvia
CHRISTIAENS, Pieter
DAENEN, Michael
HAENEN, Ken
NESLADEK, Milos
VAN DE VEN, Martin
VERMEEREN, Veronique
MICHIELS, Luc
AMELOOT, Marcel
WAGNER, Patrick
Issue Date: 2005
Publisher: Wiley
Citation: Physica Status Solidi (a), 202(11). p. 2212-2216
Abstract: A biochemical method to immobilize DNA on synthetic diamond for biosensor applications is developed. Nanocrystalline diamond is grown using microwave plasma-enhanced chemical vapour deposition. On the hydrogen-terminated surface 10-undecenoic acid is tethered photochemically under 254 nm illumination, followed by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide crosslinker-mediated attachment of amino modified DNA. The attachment is functionally confirmed by comparison of supernatant fluorescence and gel electrophoresis. The linking procedure allowed for 35 denaturation and rehybridisation steps.
URI: http://hdl.handle.net/1942/924
DOI: 10.1002/pssa.200561932
ISI #: 000231925500031
ISSN: 0031-8965
Category: A1
Type: Journal Contribution
Validation: ecoom, 2006
Appears in Collections: Research publications

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