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|Title: ||DNA attachment to nanocrystalline diamond films|
|Authors: ||WENMACKERS, Sylvia|
VAN DE VEN, Martin
|Issue Date: ||2005|
|Citation: ||Physica Status Solidi (a), 202(11). p. 2212-2216|
|Abstract: ||A biochemical method to immobilize DNA on synthetic diamond for biosensor applications is developed.
Nanocrystalline diamond is grown using microwave plasma-enhanced chemical vapour deposition. On the
hydrogen-terminated surface 10-undecenoic acid is tethered photochemically under 254 nm illumination,
followed by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide crosslinker-mediated attachment of amino
modified DNA. The attachment is functionally confirmed by comparison of supernatant fluorescence and
gel electrophoresis. The linking procedure allowed for 35 denaturation and rehybridisation steps.|
|ISI #: ||000231925500031|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2006|
|Appears in Collections: ||Research publications|
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