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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/914

Title: Head-on immobilization of DNA fragments on CVD-diamond layers
Authors: WENMACKERS, Sylvia
CHRISTIAENS, Pieter
DEFERME, Wim
HAENEN, Ken
NESLADEK, Milos
WAGNER, Patrick
VERMEEREN, Veronique
MICHIELS, Luc
VAN DE VEN, Martin
DAENEN, Michael
AMELOOT, Marcel
Wouters, J.
Naelaerts, L.
Mekhalif, Z.
Issue Date: 2005
Publisher: Trans Tech Publications
Citation: Materials Science Forum, 492-493. p. 267-272
Abstract: Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.
URI: http://hdl.handle.net/1942/914
Link to publication: http://www.scientific.net/0-87849-970-9/267/
ISI #: 000230986100044
ISSN: 0255-5476
Category: A1
Type: Journal Contribution
Validation: ecoom, 2006
Appears in Collections: Research publications

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