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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2464

Title: Covalent immobilization of DNA on CVD diamond films
Authors: WENMACKERS, Sylvia
HAENEN, Ken
NESLADEK, Milos
WAGNER, Patrick
MICHIELS, Luc
VAN DE VEN, Martin
AMELOOT, Marcel
Issue Date: 2003
Publisher: WILEY-V C H VERLAG GMBH
Citation: PHYSICA STATUS SOLIDI A-APPLIED RESEARCH, 199(1). p. 44-48
Abstract: Chemical vapour deposited (CVD) diamond is used in DNA immobilization experiments for its high stability and versatility. In this study a double-stranded, 250 base pairs long DNA fragment of the human PKU gene was covalently bound to CVD diamond films. Thymidine was used as a linker molecule for the binding in head-on configuration. The presence of surface-bound FITC-labelled DNA was confirmed by confocal fluorescence microscopy. In denaturation experiments the second, unlabelled strand of each pair was removed resulting in a loss of fluorescence. The possibility of non-specific binding of DNA to diamond can be excluded. (C) 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Notes: Limburgs Univ Centrum, Mat Res Inst, B-3590 Diepenbeek, Belgium. Linburgs Univ Centrum, Biomed Res Inst, B-3590 Diepenbeek, Belgium.Wenmackers, S, Limburgs Univ Centrum, Mat Res Inst, Wetenschapspk 1, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2464
DOI: 10.1002/pssa.200303822
ISI #: 000185422000010
ISSN: 0031-8965
Category: A1
Type: Journal Contribution
Validation: ecoom, 2004
Appears in Collections: Research publications

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