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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1626

Title: EDC-mediated DNA attachment to nanocrystalline CVD diamond films
Authors: CHRISTIAENS, Pieter
VERMEEREN, Veronique
WENMACKERS, Sylvia
DAENEN, Michael
HAENEN, Ken
NESLADEK, Milos
VAN DE VEN, Martin
AMELOOT, Marcel
MICHIELS, Luc
WAGNER, Patrick
Issue Date: 2006
Publisher: Elsevier
Citation: BIOSENSORS & BIOELECTRONICS, 22(2). p. 170-177
Abstract: Chemical vapour deposited (CVD) diamond is a very promising material for biosensor fabrication owing both to its chemical inertness and the ability to make it electrical semiconducting that allows for connection with integrated circuits. For biosensor construction, a biochemical method to immobilize nucleic acids to a diamond surface has been developed. Nanocrystalline diamond is grown using microwave plasma-enhanced chemical vapour deposition (MPECVD). After hydrogenation of the surface, 10-undecenoic acid, an ω-unsaturated fatty acid, is tethered by 254 nm photochemical attachment. This is followed by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide (EDC)-mediated attachment of amino (NH2)-modified dsDNA. The functionality of the covalently bound dsDNA molecules is confirmed by fluorescence measurements, PCR and gel electrophoresis during 35 denaturation and rehybridisation steps. The linking method after the fatty acid attachment can easily be applied to other biomolecules like antibodies and enzymes.
URI: http://hdl.handle.net/1942/1626
DOI: 10.1016/j.bios.2005.12.013
ISI #: 000240906100002
ISSN: 0956-5663
Category: A1
Type: Journal Contribution
Validation: ecoom, 2007
Appears in Collections: Research publications

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