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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14445

Title: Molecular engineering of chromophores for combined second-harmonic and two-photon fluorescence in cellular imaging
Authors: De Meulenaere, Evelien
Chen, Wei-Qiang
Van Cleuvenbergen, Stijn
Zheng, Mei-Ling
Psilodimitrakopoulos, Sotiris
Paesen, Rik
Taymans, Jean-Marc
Ameloot, Marcel
Vanderleyden, Jos
Loza-Alvarez, Pablo
Duan, Xuan-Ming
Clays, Koen
Issue Date: 2012
Publisher: ROYAL SOC CHEMISTRY
Citation: CHEMICAL SCIENCE, 3 (4), p. 984-995
Abstract: A series of chromophores with enhanced second-and third-order nonlinear optical properties were engineered for use in combined second-harmonic and two-photon fluorescence microscopy. Electronaccepting moieties imparted nonlinear optical properties to the chromophores. The electron-rich carbazole core served as a template towards one-or two-dimensional chromophores. More efficient acceptor groups (pyridinium, benzazolium, benzothiazolium) on the carbazole donor core resulted in improved second-and third-order nonlinear optical properties. A selection of these chromophores was tested in a cellular environment with a multimodal multiphoton microscope. The structural differences of the chromophores resulted in high selectivity for mitochondria or the nucleus in two-photon fluorescence and ranging from no signal to high selectivity for mitochondria in the SHG channel.
Notes: [Chen, Wei-Qiang; Zheng, Mei-Ling; Duan, Xuan-Ming] Chinese Acad Sci, Tech Inst Phys & Chem, Lab Organ NanoPhoton, Beijing 100190, Peoples R China. [De Meulenaere, Evelien; Van Cleuvenbergen, Stijn; Clays, Koen] Univ Louvain, Dept Chem, B-3001 Louvain, Belgium. [Chen, Wei-Qiang; Zheng, Mei-Ling; Duan, Xuan-Ming] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Funct Crystals & Laser Technol, Beijing 100190, Peoples R China. [De Meulenaere, Evelien; Vanderleyden, Jos] Univ Louvain, Ctr Microbial & Plant Genet, B-3001 Louvain, Belgium. [De Meulenaere, Evelien; Vanderleyden, Jos; Clays, Koen] Univ Louvain, Inst Nanoscale Phys & Chem INPAC, B-3001 Louvain, Belgium. [Psilodimitrakopoulos, Sotiris; Loza-Alvarez, Pablo] Inst Photon Sci, ICFO, Castelldefels 08860, Barcelona, Spain. [Paesen, Rik; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Taymans, Jean-Marc] Univ Louvain, Lab Neurobiol & Gene Therapy, B-3001 Louvain, Belgium.
URI: http://hdl.handle.net/1942/14445
DOI: 10.1039/c2sc00771a
ISI #: 000301122000006
ISSN: 2041-6520
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Bio-Physics
Biomedical Research Institute

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