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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14239

Title: Microglia Proliferation Is Controlled by P2X7 Receptors in a Pannexin-1-Independent Manner during Early Embryonic Spinal Cord Invasion
Authors: Rigato, Chiara
Swinnen, Nina
Buckinx, Roeland
Couillin, Isabelle
Mangin, Jean-Marie
Rigo, Jean-Michel
Legendre, Pascal
Le Corronc, Herve
Issue Date: 2012
Publisher: SOC NEUROSCIENCE
Citation: JOURNAL OF NEUROSCIENCE, 32 (34), p. 11559-11573
Abstract: Microglia are known to invade the mammalian spinal cord (SC) at an early embryonic stage. While the mechanisms underlying this early colonization of the nervous system are still unknown, we recently found that it is associated, at least partially, with the ability of microglia to proliferate at the onset of motoneuron developmental cell death and of synaptogenesis in mouse embryo (E13.5). In vitro studies have shown that the proliferation and activation of adult microglia can be influenced by the purinergic ionotropic receptor P2X7 via a coupling with Pannexin-1. By performing patch-clamp recordings in situ using a whole-mouse embryonic SC preparation, we show here that embryonic microglia already express functional P2X7R. P2X7R activation evoked a biphasic current in embryonic microglia, which is supposed to reflect large plasma membrane pore opening. However, although embryonic microglia express pannexin-1, this biphasic current was still recorded in microglia of pannexin-1 knock-out embryos, indicating that it rather reflected P2X7R intrinsic pore dilatation. More important, we found that proliferation of embryonic SC microglia, but not their activation state, depends almost entirely on P2X7R by comparing wild-type and P2X7R-/- embryos. Absence of P2X7R led also to a decrease in microglia density. Pannexin-1-/- embryos did not exhibit any difference in microglial proliferation, showing that the control of embryonic microglial proliferation by P2X7R does not depend on pannexin-1 expression. These results reveal a developmental role of P2X7R by controlling embryonic SC microglia proliferation at a critical developmental state in the SC of mouse embryos.
Notes: [Rigato, Chiara; Swinnen, Nina; Buckinx, Roeland; Mangin, Jean-Marie; Legendre, Pascal; Le Corronc, Herve] Univ Paris 06, U952, INSERM, F-75005 Paris, Ile De France, France. [Rigato, Chiara; Swinnen, Nina; Buckinx, Roeland; Mangin, Jean-Marie; Legendre, Pascal; Le Corronc, Herve] Univ Paris 06, UMR 7224, CNRS, F-75005 Paris, Ile De France, France. [Rigato, Chiara; Swinnen, Nina; Buckinx, Roeland; Mangin, Jean-Marie; Legendre, Pascal; Le Corronc, Herve] Univ Paris 06, UPMC, F-75005 Paris, Ile De France, France. [Swinnen, Nina; Rigo, Jean-Michel] Hasselt Univ, BIOMED Cell Physiol, B-3590 Diepenbeek, Belgium. [Buckinx, Roeland] Univ Antwerp, Cell Biol & Histol Lab, Antwerp, Belgium. [Couillin, Isabelle] CNRS, Transgenose Inst, UMR IEM Mol Immunol & Embryol 6218, F-45071 Orleans, France. [Le Corronc, Herve] Univ Angers, F-49000 Angers, France.
URI: http://hdl.handle.net/1942/14239
DOI: 10.1523/JNEUROSCI.1042-12.2012
ISI #: 000308140500005
ISSN: 0953-816X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Biomedical Research Institute
Physiology

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