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Molecular and Physical Plant Physiology : [127]

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1. To assess the role played by the endogenic hormone balance on developmental processes, physiological responses are studied of plants, genetically transformed on the one hand to overproduce either cytokinines or ethylene and on the other hand that possess a changed SAM-(S-adenosyl-methionine) metabolism. Several plants, each characterized by a specific transformed genetic construction are used. The effect of the transformation (cytokinine- and SAM-plants) is studied on plant development, phytohormone content and on several parameters of the photochemical and non-photochemical quenching analysis, capacity of the electron transport system, biochemical activities of the chloroplast and sugar metabolism. Analysis of transcription and translation products are performed using several molecular-biological techniques. On the plant level, gas-exchange measurements and fluorescence image analysis are used to study the physiological respons of the plants with a change in the ethylene biosynthesis. Several heavy metals are applied to induce vegetation stress and oxygen radicals. 2. The production of good quality pitfruit is still an important economic issue. To improve the assessment of the quality of apples and pears, non-destructive technology is being developed. Hereby, fluorescence induction induced by several light qualities (red, blue and UV-light) is analysed by imaging technology. Several well known quality parameters such as for instance sugar and starch content, firmness, background colour, internal breakdown and scald are analysed using physical and biochemical techniques. Analysis of secundary metabolites (phenol derivatives) with chemical methods (HPLC-MS) and enzymatic measurements support the analysis of specific fluorescence-emission patterns. Correlation studies are performed to link the non-destructive fluorescence signals with the internal quality parameters.

1. To assess the role played by the endogenic hormone balance on developmental processes, physiological responses are studied of plants, genetically transformed on the one hand to overproduce either cytokinines or ethylene and on the other hand that possess a changed SAM-(S-adenosyl-methionine) metabolism. Several plants, each characterized by a specific transformed genetic construction are used. The effect of the transformation (cytokinine- and SAM-plants) is studied on plant development, phytohormone content and on several parameters of the photochemical and non-photochemical quenching analysis, capacity of the electron transport system, biochemical activities of the chloroplast and sugar metabolism. Analysis of transcription and translation products are performed using several molecular-biological techniques. On the plant level, gas-exchange measurements and fluorescence image analysis are used to study the physiological respons of the plants with a change in the ethylene biosynthesis. Several heavy metals are applied to induce vegetation stress and oxygen radicals. 2. The production of good quality pitfruit is still an important economic issue. To improve the assessment of the quality of apples and pears, non-destructive technology is being developed. Hereby, fluorescence induction induced by several light qualities (red, blue and UV-light) is analysed by imaging technology. Several well known quality parameters such as for instance sugar and starch content, firmness, background colour, internal breakdown and scald are analysed using physical and biochemical techniques. Analysis of secundary metabolites (phenol derivatives) with chemical methods (HPLC-MS) and enzymatic measurements support the analysis of specific fluorescence-emission patterns. Correlation studies are performed to link the non-destructive fluorescence signals with the internal quality parameters.

Recent Submissions

The use of a fluorescence imaging system to study pre- and postharvest stress in fruit trees and apples

Study of transgenic tobacco plants containing the ipt-gene under control of a tetracycline-inducible promoter

Development of a chlorophyll fluorescence imaging system for the quality assessment of fruits and vegetables

Cadmium responses in Arabidopsis thaliana: a study focusing on Subcellular localization

Cytokinins affect the structure and function of the photosynthetic apparatus: a multidisciplinary approach

 

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